More and more researches focus on microRNA (miRNA) and long non-coding RNA (lncRNA) as they seem to play an important role in gene regulation. QPCR is a method also suited to quantify miRNA and lncRNA (Lui 2013, Jiang 2014) and with primaQUANT miRNA we have designed a special qPCR product for you.
For more detailed information about the use of qPCR for miRNA oder lncRNA analysis please see:
Liu, S.-P., Yang, J.-X., Cao, D.-Y., & Shen, K. (2013). Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials. Cancer Biology & Medicine, 10(3), 138–141. https://doi.org/10.7497/j.issn.2095-3941.2013.03.003
Jiang, X.-Y., & Ning, Q.-L. (2014). Expression profiling of long noncoding RNAs and the dynamic changes of lncRNA-NR024118 and Cdkn1c in angiotensin II-treated cardiac fibroblasts. International Journal of Clinical and Experimental Pathology, 7(4), 1325–1336. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/24817929
Mou, G., Wang, K., Xu, D., & Zhou, G. (n.d.). Evaluation of Three RT-qPCR-Based miRNA Detection Methods Using Seven Rice miRNAs. https://doi.org/10.1271/bbb.130192
Wan, G., Lim, Q. E., & Too, H.-P. (2010). High-performance quantification of mature microRNAs by real-time RT-PCR using deoxyuridine-incorporated oligonucleotides and hemi-nested primers. RNA (New York, N.Y.), 16(7), 1436–1445. https://doi.org/10.1261/rna.2001610
Androvic, P., Valihrach, L., Elling, J., Sjoback, R., & Kubista, M. (2017). Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Research, 45(15). https://doi.org/10.1093/nar/gkx588