Though a qPCR assay mostly needs to be optimised individually, there are a few standard protocols which may be helpful to use at the beginning.

If you choose a ready-to-use master mix  like our primaQUANT mix, all you have to do is to add your primers and the cDNA template. With our qPCR Mastermix Calculator the calculation of the qPCR assay becomes a pretty easy task and takes only a few seconds.

For an overview of existing standard protocols in 96- and 384-format please click here.