It is a myth that one cannot switch to another master mix within a project or series of experiments! The only prerequisit to switch to a better product is that you have normalised your assay. (Which one should do anyway).
Our recommendations for a successful switch:
- Run your negative and positive controls with both mixes in parallel. This allows you to directly compare their performances.
- Have a close look at the fluorescence curve – are there signs for a PCR inhibition?
- Run your assay with a cDNA dilution series to compare the dynamic range of different master mixes
- Check if the setup of your cycler fits the requirements of the master mixes tested
For further information please visit our qPCR Knowledge Center – How to test and optimise a qPCR .