extrAXON Spin-Column Extraction Kits
Extraction of DNA, RNA, and Plasmid DNA
DNA Extraction
from cell culture, tissue (fresh, frozen or fixated), blood and other liquids
RNA Extraction
from cell culture and tissue (fresh, frozen or fixated)
Plasmid DNA Extraction
from bacteria
DNA Gel-Out Kit
for isolation of DNA fragments (genomic DNA, plasmid DNA, PCR fragments) from agarose gels
DNA Cleanup Kit
for isolation of DNA (genomic DNA, plasmid DNA, PCR fragments) from liquid samples like PCR reactions
extrAXON DNA Tissue Kit
The extrAXON DNA Tissue Spin-Column Kit is suited for (g)DNA extraction from blood and other liquid samples, cell culture, insects, mousetails, hair, tissue (fresh, frozen, formalin-preserved, and paraffin-embedded). An advanced buffer system guarantees a high yield and purity of the DNA.
Ready-to-use kit including RNAse and all other enzymes needed
High yield of up to 50 µg DNA
High quality and purity of the DNA
Advanced buffer for difficult samples
Additional information
Tissue (fresh or frozen): 1 -30 mg
Formalin-preserved tissue: up to 30 mg
Paraffin-embedded tissue: up to 30 mg
Cell culture: 10^3 to 10^7 cells
Liquid samples : 1 – 5 mL
Hair : 10 – 30 mg
Insects : 1 – 30 mg
Tails (mouse – and rat) : up to 30 mg
For research only
Fast protocol: 10-12 min (without lysis)
Optional 30 – 40 min mechanical homogenisation
5 min RNase digest (recommended)
Typical yield of the extrAXON DNA tissue kit
| Sample Material | Input Quantity | Elution Volume | DNA Concentration | A260 / A280 | Yield |
|---|---|---|---|---|---|
| Rat liver | 30 mg | 200 μl | 235.2 ng/ μl | > 1.86 | 47 μg |
| Rat skeletal muscle | 20 mg | 200 μl | 50.5 ng/ μl | > 1.80 | 10.1 μg |
| Rat heart | 20 mg | 200 μl | 123.5 ng/ μl | > 1.84 | 24.7 μg |
| Yellow adipose tissue | 30 mg | 200 μl | 49 ng/ μl | > 1.80 | 9.8 μg |
| Brown adipose tissue | 30 mg | 200 μl | 116.7 ng/ μl | > 1.88 | 23.3 μg |
| Rat kidney | 20 mg | 200 μl | 183.4 ng/ μl | > 1.79 | 36.7 μg |
| HT29 cell culture | 1 x 10^5 | 200 μl | 51.5 ng/ μl | > 1.81 | 10.3 μg |
| HCT116 cell culture | 3 x 10^6 | 200 μl | 60 ng/ μl | > 1.71 | 12.0 μg |
| Rat brain | 20 mg | 200 μl | 9.4 ng/ μl | > 1.69 | 1.88 μg |
| Insects | 2.7 mg | 30 μl | 12.9 ng/ μl | > 1.65 | 0.39 μg |
Typical lysis time of the extrAXON DNA tissue kit
| Sample Material | Digestion in a block heater, no homogenization, periodical mixing | Digestion in a thermomixer | Liquid nitrogen homogenization | Approximate lysis time |
|---|---|---|---|---|
| Rat liver | 3 – 6 h | 2 – 4 h | 1 – 2 h | min 1 h, max 6 h |
| Rat skeletal muscle | 2 – 3 h | 2 h | ≤ 1 h | min 0.5 h, max 3 h |
| Rat heart | 2 – 3 h | 1 – 1.5 h | ≤ 1 h | min 0.5 h, max 3 h |
| Yellow adipose tissue | 1 – 1.5 h | 1 h | 0.5 h | min 0.5 h, max 1.5 h |
| Brown adipose tissue | 1 – 1.5 h | 1 h | 0.5 h | min 0.5 h, max 1.5 h |
| Rat kidney | 1.5 – 3 h | 0.5 – 2 h | 0.5 – 1.5 h | min 0.5 h, max 3 h |
| HT29 cell culture | 0.5 – 1 h | 5 – 30 min | No data | min 5 min, max 1 h |
| HCT116 cell culture | 0.5 – 1 h | 5 – 30 min | No data | min 5 min, max 1 h |
| Rat brain | 0.5 – 1 h | 0.5 – 1 h | No data | min 0.5 h, max 1 h |
| Insects | No data | 1 – 2 h | 0.5 – 2 h | min 0.5 h, max 2 h |
Item
| Item | Pack size |
|---|---|
| SL-11003314 | 50 extractions |
| SL-11003316 | 250 extractions |
| Contents |
|---|
| extrAXON DNA tissue kit incl. all enzymes. Ethanol has to be provided by the customer for safety reasons. |
Downloads
extrAXON Total RNA Kit
extrAXON total RNA spin-column kit for fast extraction of RNA from up to 30 mg tissue (fresh or frozen) or up to 10^7 cells. The kit is perfectly suited for RT-PCR, RT-qPCR, northern blotting, cDNA synthesis, primer extension, RNA sequencing and microarrays.
Ready-to-use kit including DNase I and all other enzymes needed
High yield of up to 230 µg RNA/column
High quality and purity of the RNA
Advanced buffer for difficult samples
Additional information
For fresh and frozen tissue (up to 30 mg)
Suited for tissue preserved with RNase-inactivating buffer (e.g. RNAlater®, Ambion), up to 30 mg
Cell culture (up to 10^7 cells)
For research only
Fast extraction (10-12 min without lysis)
Optional homogenisation for 15 – 30 in liquid nitrogen
Optional mechanical homogenisation for 15 – 20 min
5 min DNase I digest (recommended)
Typical yield of the extrAXON total RNA kit
| Sample Material | Quantity | Yield |
|---|---|---|
| HEK293 | 10^6 cells | 5 µg |
| HeLa | 10^6 cells | 7 µg |
| Liver tissue | 10 mg | 75 µg |
| Heart tissue | 5 mg | 10 µg |
| Brain tissue | 5 mg | 5 µg |
Item
| Item | Pack size |
|---|---|
| SL-11003319 | 50 extractions |
| SL-11003320 | 250 extractions |
| Content |
|---|
| extrAXON Total RNA Kit incl. all enzymes. Ethanol has to be provided by the user for safety reasons. |
Downloads
extrAXON Plasmid Mini Kit
extrAXON Plasmid Mini Spin-column kit for fast extraction of plasmid DNA from bacteria. Advanced buffer system guarantees high yield and quality of the DNA. Perfectly suited for cloning.
Ready-to-use kit including all enzymes needed
Suited for low-copy and high-copy plasmids
High yield of up to 60 µg plasmid DNA/column
High quality and purity of the plasmid DNA
Advanced buffer system works also with difficult samples
Suited for fresh and frozen bacteria
BalticBlue indicator allows easy visual control of the lysis
Additional information
For fresh and frozen bacteria (up to 10 mL/column)
For research only
Only 25 min/run
Item
| Item | Pack size |
|---|---|
| SL-11003321 | 250 extractions |
| Contents |
|---|
| extrAXON Plasmid DNA Kit incl. all enzymes. Ethanol has to be provided by the user for safety reasons. |
Downloads
extrAXON Plasmid Midi Kit
Plasmid Midi Kit for bacteria. Advanced buffer system for high yield and purity of the DNA.
Ready-to-use kit including all enzymes needed
Suited for low-copy and high-copy plasmids
High yield of up to 600 µg plasmid DNA/column
High quality and purity of the plasmid DNA
Advanced buffer system works also with difficult samples
Suited for fresh and frozen bacteria
BalticBlue indicator allows visual control of lysis
Suited for up to 300 mL bacterial culture
Additional Information
Suited for fresh and frozen bacteria
Suited for up to 300 mL bacterial culture
Binding capacity up to 600 µg/column
For research only
70 min for extraction
60 min for DNA precipitation and clean-up
Item
| Item | Pack size |
|---|---|
| SL-11003322 | 25 extractions |
| Content |
|---|
| extrAXON Plasmid DNA Midi Kit including enzymes. Ethanol has to be supplied by the customer for safety reasons. |
Downloads
extrAXON DNA Clean-Up Kit
extrAXON kit for PCR clean-up. Removes inhibitors of downstream processes like nucleases, primers, inhibitors, detergents, restriction enzymes, polymerases, ions, salts, and oils.
Ready-to-use kit for DNA fragments from 50 bp to 20 kbp
Removes nucleases, salts, nucleotides, and proteins
High yield of up to 40 µg/column
Advanced buffer system for difficult samples
Suited for clean-up of up to 200 µL PCR reactions
Binding capacity 40 µg
Including loading buffer for agarose gels
Additional Information
PCR reactions
Restriction digest
Enrichment of PCR fragments/DNA
Cleanup of PCR-/DNA samples of poor quality
For research only
10 min for 6 samples
Item
| Item | Pack size |
|---|---|
| SL-11003322 | 25 extractions |
| Content |
|---|
| extrAXON Plasmid DNA Midi Kit including all enzymes. Ethanol has to be added by the user for safety reasons. |
Downloads
extrAXON DNA Gel-Out Kit
Our extrAXON DNA Gel-Out Spin-column Kit for isolation and concentrating from DNA and PCR-fragments from agarose gels. Removes intercaling dyes, salts and other undesired molecules.
Ready-to-use kit for PCR fragments and DNA from 50 bp to 30kb
High yield of up to 25 µg DNA/column
High recovery of 70-95%
Capacity of up to 300 mg gel/column
Additional information
Suited for up to 300 mg agarose
High binding capacity of up to 25 µg/column
For research only
Fast protocol: 6 samples in 16 min
Item
| Item | Pack size |
|---|---|
| SL-11003313 | 50 extractions |
| Content |
|---|
| extrAXON Plasmid DNA Midi Kit including all enzymes. Ethanol has to be added by the user for safety reasons. |
Downloads
Ihre Ansprechpartner
Manfred Lux
Sales Manager Germany
Your Product Specialist for KingFisher Accessories, LIQUIDATOR96, NGS, and qPCR
+49 (152) 3361 3268
ml@steinbrenner-laborsysteme.de
Jan Winter
Product Manager Genomics
PCR, qPCR, Magnetic Beads & CRISPR/Cas9
+49 (152) 3361 2537
jw@steinbrenner-laborsysteme.de