extrAXON Spin-Column Extraction Kits

Extraction of DNA, RNA, and Plasmid DNA

DNA Extraction

from cell culture, tissue (fresh, frozen or fixated), blood and other liquids

RNA Extraction

from cell culture and tissue (fresh, frozen or fixated)

Plasmid DNA Extraction

from bacteria

DNA Gel-Out Kit

for isolation of DNA fragments (genomic DNA, plasmid DNA, PCR fragments) from agarose gels

DNA Cleanup Kit

for isolation of DNA (genomic DNA, plasmid DNA, PCR fragments) from liquid samples like PCR reactions

DNA Extraction from Blood, Cell Culture, Insects, Tissue and Liquid Biological Samples

extrAXON DNA Tissue Kit

The extrAXON DNA Tissue Spin-Column Kit is suited for (g)DNA extraction from blood and other liquid samples, cell culture, insects, mousetails, hair, tissue (fresh, frozen, formalin-preserved, and paraffin-embedded). An advanced buffer system guarantees a high yield and purity of the DNA.

  • Ready-to-use kit including RNAse and all other enzymes needed
  • High yield of up to 50 µg DNA
  • High quality and purity of the DNA
  • Advanced buffer for difficult samples
Additional information
Intended Use
  • Tissue (fresh or frozen): 1 -30 mg
  • Formalin-preserved tissue: up to 30 mg
  • Paraffin-embedded tissue: up to 30 mg
  • Cell culture: 10^3 to 10^7 cells
  • Liquid samples : 1 – 5 mL
  • Hair : 10 – 30 mg
  • Insects : 1 – 30 mg
  • Tails (mouse – and rat) : up to 30 mg
  • For research only
Processing Time
  • Fast protocol: 10-12 min (without lysis)
  • Optional 30 – 40 min mechanical homogenisation
  • 5 min RNase digest (recommended)
Typical yield of the extrAXON DNA tissue kit
Typical lysis time of the extrAXON DNA tissue kit
Item
ItemPack size
SL-1100331450 extractions
SL-11003316250 extractions
Contents
extrAXON DNA tissue kit incl. all enzymes. Ethanol has to be provided by the customer for safety reasons.
Downloads
extrAXON DNA Tissue Kit - Manual
 319.12 KB
 21. January 2019
Version 012018

Fast RNA extraction from cell culture and tissue

extrAXON Total RNA Kit

extrAXON total RNA spin-column kit for fast extraction of RNA from up to 30 mg tissue (fresh or frozen) or up to 10^7 cells. The kit is perfectly suited for RT-PCR, RT-qPCR, northern blotting, cDNA synthesis, primer extension, RNA sequencing and microarrays.

  • Ready-to-use kit including DNase I and all other enzymes needed
  • High yield of up to 230 µg RNA/column
  • High quality and purity of the RNA
  • Advanced buffer for difficult samples
Additional information
Intended Use
  • For fresh and frozen tissue (up to  30 mg)
  • Suited for tissue preserved with RNase-inactivating buffer (e.g. RNAlater®, Ambion), up to 30 mg
  • Cell culture (up to 10^7 cells)
  • For research only
Processing Time
  • Fast extraction (10-12 min without lysis)
  • Optional homogenisation for 15 – 30 in liquid nitrogen
  • Optional mechanical homogenisation for 15 – 20 min
  • 5 min DNase I digest (recommended)
Typical yield of the extrAXON total RNA kit
Item
ItemPack size
SL-1100331950 extractions
SL-11003320250 extractions
Content
extrAXON Total RNA Kit incl. all enzymes. Ethanol has to be provided by the user for safety reasons.
Downloads
extrAXON Total RNA Kit - Manual
 464.28 KB
 21. January 2019
Version 062018

Fast extraction of plasmid DNA from bacteria

extrAXON Plasmid Mini Kit

extrAXON Plasmid Mini Spin-column kit for fast extraction of plasmid DNA from bacteria. Advanced buffer system guarantees high yield and quality of the DNA. Perfectly suited for cloning.

  • Ready-to-use kit including all enzymes needed
  • Suited for low-copy and high-copy plasmids
  • High yield of up to 60 µg plasmid DNA/column
  • High quality and purity of the plasmid DNA
  • Advanced buffer system works also with difficult samples
  • Suited for fresh and frozen bacteria
  • BalticBlue indicator allows easy visual control of the lysis
Additional information
Intended Use
  • For fresh and frozen bacteria (up to 10 mL/column)
  • For research only

Processing Time
  • Only 25 min/run
Item
ItemPack size
SL-11003321250 extractions
Contents
extrAXON Plasmid DNA Kit incl. all enzymes. Ethanol has to be provided by the user for safety reasons.
Downloads
extrAXON Plasmid Mini Kit - Manual
 318.03 KB
 21. January 2019
Version 062018

extrAXON Plasmid Midi Kit

Plasmid Midi Kit for bacteria. Advanced buffer system for high yield and purity of the DNA.

  • Ready-to-use kit including all enzymes needed
  • Suited for low-copy and high-copy plasmids
  • High yield of up to 600 µg plasmid DNA/column
  • High quality and purity of the plasmid DNA
  • Advanced buffer system works also with difficult samples
  • Suited for fresh and frozen bacteria
  • BalticBlue indicator allows visual control of lysis
  • Suited for up to 300 mL bacterial culture
Additional Information
Intended Use
  • Suited for fresh and frozen bacteria
  • Suited for up to 300 mL bacterial culture
  • Binding capacity up to 600 µg/column
  • For research only
Processing Time
  • 70 min for extraction
  • 60 min for DNA precipitation and clean-up
Item
ItemPack size
SL-1100332225 extractions
Content
extrAXON Plasmid DNA Midi Kit including enzymes. Ethanol has to be supplied by the customer for safety reasons.
Downloads
extrAXON Plasmid Midi Kit - Manual
 126.54 KB
 21. January 2019
Version 062018

Fast extraction of DNA- and PCR fragments

extrAXON DNA Clean-Up Kit

extrAXON kit for PCR clean-up. Removes inhibitors of downstream processes like nucleases, primers, inhibitors, detergents, restriction enzymes, polymerases, ions, salts, and oils.

  • Ready-to-use kit for DNA fragments from 50 bp to 20 kbp
  • Removes nucleases, salts, nucleotides, and proteins
  • High yield of up to 40 µg/column
  • Advanced buffer system for difficult samples
  • Suited for clean-up of up to 200 µL PCR reactions
  • Binding capacity 40 µg
  • Including loading buffer for agarose gels
Additional Information
Intended Use
  • PCR reactions
  • Restriction digest
  • Enrichment of PCR fragments/DNA
  • Cleanup of  PCR-/DNA samples of poor quality
  • For research only
Processing Time
  • 10 min for 6 samples
Item
ItemPack size
SL-1100332225 extractions
Content
extrAXON Plasmid DNA Midi Kit including all enzymes. Ethanol has to be added by the user for safety reasons.
Downloads
extrAXON Plasmid Midi Kit - Manual
 126.54 KB
 21. January 2019
Version 062018

extrAXON DNA Gel-Out Kit

Our extrAXON DNA Gel-Out Spin-column Kit for isolation and concentrating from DNA and PCR-fragments from agarose gels. Removes intercaling dyes, salts and other undesired molecules.

  • Ready-to-use kit for PCR fragments and DNA from 50 bp to 30kb
  • High yield of up to 25 µg DNA/column
  • High recovery of 70-95%
  • Capacity of up to 300 mg gel/column
Additional information
Intended Use
  • Suited for up to 300 mg agarose
  • High binding capacity of up to 25 µg/column
  • For research only
Processing Time
  • Fast protocol: 6 samples in 16 min
Item
ItemPack size
SL-1100331350 extractions
Content
extrAXON Plasmid DNA Midi Kit including all enzymes. Ethanol has to be added by the user for safety reasons.
Downloads
extrAXON DNA Gel-Out Kit - Manual
 170.59 KB
 21. January 2019
Version 062018

Ihre Ansprechpartner

Manfred Lux

Sales Manager Germany

Your Product Specialist for KingFisher Accessories, LIQUIDATOR96, NGS, and qPCR

+49 (152) 3361 3268

ml@steinbrenner-laborsysteme.de

Jan Winter

Product Manager Genomics

PCR, qPCR, Magnetic Beads & CRISPR/Cas9

+49 (152) 3361 2537

jw@steinbrenner-laborsysteme.de