primaQUANT qPCR Master Mixes with SYBRGreen for miRNA

Excellent Performance and superior Sensitivity

Our primaQUANT miRNA qPCR master mix with SYBRGreen is especially designed for miRNA expression analysis.

Due to an extremely active hotstart polymerase and an optimised buffer system, primaQUANT miRNA detects even low abundant miRNA very reliably. For more convenient and safe pipetting, primaQUANT miRNA is now optionally available in blue.

Free samples available. Please put primaQUANT miRNA to the test and enjoy its great performance!

 

Our primaQUANT miRNA qPCR master mixes are priced very attractively. In addition, they also require very low quantities of samples and primers.

  • Optimised for miRNA
  • High sensitivity
  • Highly efficient polymerase saves costs and sample material
  • Excellent performance and reproducibility
  • Safe and userfriendly packaging: 1.25 mL tubes for reduced freeze-thaw cycles
  • Available with/without ROX, and with reduced ROX concentration (low ROX)
primaQUANT miRNA

Due to a superior sensitivity primaQUANT miRNA even detects low exprimed miRNA very reliably.

primaQUANT miRNA überzeugt mit einer sehr guten Effizienz über die gesamte Dynamic Range.

qPCR in 96-well format

Reagent Volume
2x primaQUANT miRNA Mastermix 12.5 µL
PCR Primer Mix (5 uM) 1 µL
cDNA Template 6 µL
RNAse-free Water 5.5 µL
Total 25 µL

qPCR in 384-well format

Reagent Volume
2x primaQUANT miRNA Mastermix 5 µL
PCR Primer Mix (2.5 uM) 1 µL
cDNA Template 3 µL
RNAse-free Water 1 µL
Total 10 µL

qPCR Settings

Step Time Temperature
Initial Activation 2 min 95 ℃
Denaturing (jeder Zyklus) 10 s 95 ℃
Annealing/Extension combines (each cycle) 60 s 60 ℃

SYBRGreen miRNA master mixes

primaQUANT miRNA without ROX
Item Pack Size
SL-9904-5mL 5 mL
SL-9904-10mL 10 mL
SL-9904-20mL 20 mL
SL-9904-50mL 50 mL
SL-9904B 5 mL, 10 mL, 20 mL, with blue dye
  • Ready-to-use 2x master mix with SYBRGreen
  • Optimised for ROCHE LightCycler®
  • Suited for GC-rich templates
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primaQUANT miRNA master mix with ROX
Item Pack Size
SL-9914-5mL 5 mL
SL-9914-10mL 10 mL
SL-9914-20mL 20 mL
SL-9914-50mL 50 mL
SL-9914B 5 mL, 10 mL, 20 mL, with blue dye
  • Ready-to-use 2x master mix with SYBRGreen
  • Optimised for ABI™ und Bio-Rad®
  • Suited for GC-rich templates
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primaQUANT miRNA Master Mix with reduced ROX concentration
Item Pack Size
On Request 5 mL, 10 mL, 20 mL
  • Ready-to-use 2x Master Mix with SYBRGreen
  • Optimised for ABI™ Quantstudio 6/7™
  • Suited for GC-rich templates
Request Sample
Request Quote

Further information and downloads

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Your product specialist

Jan Winter

Product Manager Genomics

PCR, qPCR, Magnetic Beads & CRISPR/Cas9

+49 (152) 3361 2537

jw@steinbrenner-laborsysteme.de