primaQUANT qPCR Master Mixes with SYBRGreen for miRNA

Excellent Performance and superior Sensitivity

Our primaQUANT miRNA qPCR master mix with SYBRGreen is especially designed for miRNA expression analysis.

Due to an extremely active hotstart polymerase and an optimised buffer system, primaQUANT miRNA detects even low abundant miRNA very reliably. For more convenient and safe pipetting, primaQUANT miRNA is now optionally available in blue.

Free samples available. Please put primaQUANT miRNA to the test and enjoy its great performance!

 

Our primaQUANT miRNA qPCR master mixes are priced very attractively. In addition, they also require very low quantities of samples and primers.

  • Optimised for miRNA
  • High sensitivity
  • Highly efficient polymerase saves costs and sample material
  • Excellent performance and reproducibility
  • Safe and userfriendly packaging: 1.25 mL tubes for reduced freeze-thaw cycles
  • Available with/without ROX, and with reduced ROX concentration (low ROX)
primaQUANT miRNA

Due to a superior sensitivity primaQUANT miRNA even detects low exprimed miRNA very reliably.

primaQUANT miRNA überzeugt mit einer sehr guten Effizienz über die gesamte Dynamic Range.

qPCR in 96-well format

ReagentVolume
2x primaQUANT miRNA Mastermix12.5 µL
PCR Primer Mix (5 uM)1 µL
cDNA Template6 µL
RNAse-free Water5.5 µL
Total25 µL

qPCR in 384-well format

ReagentVolume
2x primaQUANT miRNA Mastermix5 µL
PCR Primer Mix (2.5 uM)1 µL
cDNA Template3 µL
RNAse-free Water1 µL
Total10 µL

qPCR Settings

StepTimeTemperature
Initial Activation2 min95 ℃
Denaturing (jeder Zyklus)10 s95 ℃
Annealing/Extension combines (each cycle)60 s60 ℃

SYBRGreen miRNA master mixes

primaQUANT miRNA without ROX
ItemPack Size
SL-9904-5mL5 mL
SL-9904-10mL10 mL
SL-9904-20mL20 mL
SL-9904-50mL50 mL
SL-9904B5 mL, 10 mL, 20 mL, with blue dye
  • Ready-to-use 2x master mix with SYBRGreen
  • Optimised for ROCHE LightCycler®
  • Suited for GC-rich templates
primaQUANT miRNA master mix with ROX
ItemPack Size
SL-9914-5mL5 mL
SL-9914-10mL10 mL
SL-9914-20mL20 mL
SL-9914-50mL50 mL
SL-9914B5 mL, 10 mL, 20 mL, with blue dye
  • Ready-to-use 2x master mix with SYBRGreen
  • Optimised for ABI™ und Bio-Rad®
  • Suited for GC-rich templates
primaQUANT miRNA Master Mix with reduced ROX concentration
ItemPack Size
On Request5 mL, 10 mL, 20 mL
  • Ready-to-use 2x Master Mix with SYBRGreen
  • Optimised for ABI™ Quantstudio 6/7™
  • Suited for GC-rich templates

Further information and downloads

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Your product specialist

Jan Winter

Product Manager Genomics

PCR, qPCR, Magnetic Beads & CRISPR/Cas9

+49 (152) 3361 2537

jw@steinbrenner-laborsysteme.de