primaQUANT real-time PCR (qPCR) Master Mixes for Probes (e.g. TaqMan) – Superior Sensitivity and Reproducibility

Our primaQUANT qPCR master mixes for

  • Probe-based qPCR (e.g. TaqMan™ or Lightcycler® probes)
  • Endpoint analysis
  • FRET probes
  • Fast PCR
  • Multiplex PCR

They have proven their convincing performance many times. Users are thrilled by their high sensitivity and low detection limit of up to < 10 pg. At the same time, the primaQUANT mixes are very robust and deliver reproducible results with most common protocols.

Now also in blue – for perfect visible control during pipetting. The dye does not interfere with the qPCR reaction and does not require any changes to the setup.

  • Suited for probe-based assays (TaqMan™ or Lightcycler® probes)
  • Detection limit better than 10 pg DNA
  • Robust and reliable performance without much modification
  • Highly efficient polymerase saves costs and samples
  • Reproducible results
  • Safe and convenient packaging format (1.25 mL tubes)
  • Versions with and without ROX and with low ROX available

Our qPCR master mixes for probe-based assays

primaQUANT Probe Master Mix without ROX
Item Pack Size
SL-9802-5mL 5 mL
SL-9802-10mL 10 mL
SL-9802-20mL 20 mL
SL-9802B-x Blue version, x= 5mL, 10 mL, 20mL
  • Master mix without ROX
  • Suited for ROCHE LightCycler™
  • Ready-to-use master 2 x mix for hydrolysis probes
  • Suited for GC-rich templates
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PrimaQUANT qPCR Master Mixes with ROX
Item Pack Size
SL-9812-5mL 5 mL
SL-9812-10mL 10 mL
SL-9812-20mL 20 mL
SL-9812B-x Blue version, x= 5mL, 10 mL, 20mL
  • Suited for cycler from ABI™ and Bio-Rad® and others
  • Ready-to-use 2x master mix
  • Suited for GC-rich templates
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primaQUANT probe master mix with reduced ROX concentration

Item Pack Size
On Request 5 mL, 10 mL, 20 mL
  • Suited for ABI™ Quantstudio 6/7™ and others
  • Ready-to-use 2x master mix for hydrolysis probes
  • Suited for GC-rich templates
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Information and Downloads

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primaQUANT 1Step RT-qPCR Manual 899.33 KB 805 downloads

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primaQUANT Probe SL-9802 1.21 MB 3988 downloads

primaQUANT 2x Probe Master Mix; no ROX - Manual and Protocol ...
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primaQUANT Probe SL-9802B 1.21 MB 3912 downloads

primaQUANT 2x Probe Blue Master Mix; no ROX - Manual and Protocol ...
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primaQUANT Probe SL-9812 1.21 MB 3928 downloads

primaQUANT 2x Probe Master Mix; high ROX - Manual and Protocol ...
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primaQUANT Probe SL-9812B 1.22 MB 3914 downloads

primaQUANT 2x Probe Blue Master Mix; high ROX - Manual and Protocol ...
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primaQUANT Probe SL-9813 1.22 MB 3934 downloads

primaQUANT 2x Probe Master Mix; low ROX - Manual and Protocol ...
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primaQUANT Probe SL-9813B 1.22 MB 3879 downloads

primaQUANT 2x Probe Blue Master Mix; low ROX - Manual and Protocol ...
    Probe qPCR Mastermix Calculator
     33.48 KB
     16. April 2019
    Version 1.0
    qPCR Efficiency Calculator EN
     97.77 KB
     16. April 2019
    Version 1.1

    Your Product Specialist

    Jan Winter

    Product Manager Genomics

    Application specialist for PCR, qPCR, Magnetic Beads & CRISPR/Cas9

    +49 (152) 3361 2537

    jw@steinbrenner-laborsysteme.de