qPCR Knowledge Center

Welcome to our “Knowledge Center Quantitative PCR (qPCR)”

More and more labs use quantitative PCR (qPCR) – also known as Real-time PCR (qRT-PCR or RT-qPCR) – as an essential tool for a variety of applications. In this knowledge center, we´ve put together a bunch of facts and how-to-information that will assure that you get perfect results with your qPCR. Please study the shortlist of topics:

Various protocols for assays in 96-/384-well plates
Tips to optimise your qPCR assays
FAQ related to qPCR
Our free Setup-Calculator for qPCR
Comparison of our primaQUANT master mix with other products
Examples of applications including protocols (e.g. miRNA)
Useful tools for qPCR

Overview

Frequently Asked Questions

Basic information and frequently asked questions around qPCR

qPCR Calculator Tool

Our qPCR calculator makes it easy to calculate your qPCR setup and the primer efficiency of your assay.

Testing and Optimisation of qPCR

A qPCR assay requires certain steps for optimisation and testing. We have put together for you a guide that provides elementary and advanced How-to´s.