Welcome to our “Knowledge Center Quantitative PCR (qPCR)”

Nowadays quantitative PCR (qPCR) – also known as Real-time PCR (qRT-PCR or RT-qPCR)  – is an essential tool for a variety of applications. In this knowledge center we have put together a bunch of facts and how-to-information that will help you to get perfect results with your qPCR. Please study the shortlist of topics:

  • Various protocols for assays in 96-/384-well plates
  • Tips to optimise your qPCR assays
  • FAQ related to qPCR
  • Our free Setup-Calculator for qPCR
  • Comparison of our primaQUANT master mix with other products
  • Examples of applications including protocols (e.g. miRNA)
  • Useful tools for qPCR

Overview

Frequently Asked Questions

Basic information and frequently asked questions around qPCR

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qPCR Calculator Tool

Our qPCR calculator makes it easy to calculate your qPCR setup and the primer efficiency of your assay.

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Testing and Optimisation of qPCR

A qPCR assay requires certain steps for optimisation and testing. We have put together for you a guide that provides elementary and advanced How-to´s.

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Standard Protocols for qPCR

You are a newcomer to qPCR and/or are looking for a proven protocol?

Then choose one of our standard protocols for your start!

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Software and Tools for qPCR

In this section, you´ll find an overview of common tools for qPCR analysis and primer design, also for miRNA.

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ROX-Compatibility

Does your qPCR-machine need ROX as reference dye? Here you´ll find the answer!

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Your Product Specialist

Jan Winter

Product Manager Genomics

PCR, qPCR, Magnetic Beads & CRISPR/Cas9

+49 (152) 3361 2537

jw@steinbrenner-laborsysteme.de