Software and Tools for qPCR
Little Helper Tools
qPCR Efficiency Calculator – Tool to calculate qPCR primer efficiencies using serial dilutions
qPCR Calculator SYBRGreen – Tool for the calculations of required reagents in a SYBR Green / Eva Green assay
qPCR Calculator Probe – Tool for the calculation of reagents for probe-based assays
Primer Design
PrimerBlast – Online Design Tool
Primer3 – Online Design Tool
PrimerQuest – Online Design Tool
QuantPrimer – Online Design Tool
rtPrimerDB – data base for qPCR primer
primerBank – data base for qPCR primer
Eurofins PrimerDesign – Online Design Tool
OpenPrimeR – R Bioconducter Package
miRNA / lncRNA Design
PrimerBlast – Online Design Tool
miRNA PrimerDesign – Online Design Tool, TaqMan only
miPrimer – Online Design Tool
miRprimer – Online Design tool für miRNA Primer
miRBase – data base for miRNA
qPCR Analysis
qBase Plus – Commercial Analysis Software
Cy0 Method – Online Analysis
DART-PCR – EXCEL Analysis Sheet
LinRegPCR – online design tool for miRNA primer
MAKERGAUL – analysis software
qpcR – analysis software
Important Publications
Bustin, S., & Huggett, J. (2017). qPCR primer design revisited. Biomolecular Detection and Quantification, 14, 19–28. https://doi.org/10.1016/j.bdq.2017.11.001
Radonić, A., Thulke, S., Mackay, I. M., Landt, O., Siegert, W., & Nitsche, A. (2004). Guideline to reference gene selection for quantitative real-time PCR. https://www.sciencedirect.com/science/article/pii/S0006291X03025646
Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., … Wittwer, C. T. (2009). The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry, 55(4), 611–622. https://doi.org/10.1373/clinchem.2008.112797
Pabinger, S., Rödiger, S., Kriegner, A., Vierlinger, K., & Weinhäusel, A. (2014). A survey of tools for the analysis of quantitative PCR (qPCR) data. Biomolecular Detection and Quantification, 1(1), 23–33. https://doi.org/10.1016/j.bdq.2014.08.002
Your Product Specialist
Jan Winter
Product Manager Genomics
PCR, qPCR, Magnetic Beads & CRISPR/Cas9
+49 (152) 3361 2537
jw@steinbrenner-laborsysteme.de