ROX compatibility of real-time qPCR machines

ROX is a non-intercalating reference dye, which can be added to the qPCR reaction when a compatible qPCR cycler is used. ROX can be used to normalize differences in the reaction volume, however, is not necessary in all cases.

Please check the table below if your qPCR cycler is ready to use ROX and at which concentration (low or high ROX).

Applied Biosystems

qPCR CyclerROX necessary?
StepOne™ Real-Time PCR Systemhigh ROX
StepOnePlus™ Real-Time PCR Systemhigh ROX
7500 Real-Time PCR Systemlow ROX
7500 Fast Real-Time PCR Systemlow ROX
7500 Fast Dx Real-Time PCR Instrumentlow ROX
7500 Real-Time PCR System for Human Identificationlow ROX
7300 Real-Time PCR SystemHigh ROX
Viia™ 7 Real-Time PCR Systemlow ROX
7900HT Fast Real-Time PCR Systemhigh ROX
OpenArray® Real-Time PCR Platformhigh ROX
QuantStudio™ 12K Flex systemlow ROX
PRISM® 7000 Sequencing Detection Systemhigh ROX
PRISM® 7700 Sequencing Detection Systemhigh ROX
PRISM® 7900 Sequencing Detection Systemhigh ROX
Gene Amp 5700high ROX
Quantstudio™ 3low Rox
Quantstudio™ 5low Rox
Quantstudio™ 6low Rox
Quantstudio™ 7low Rox

Bio-Rad

qPCR CyclerROX necessary?
CFX96 Touch™ Real-Time PCR Detection Systemno ROX
CFX384 Touch™ Real-Time PCR Detection Systemno ROX
Chromo4™ Four-Color Real-Time Detectorno ROX
CFX Connect™ Real-Time PCR Detection Systemno ROX
iQ5 Real-Time PCR Detection Systemno ROX
MiniOpticon™ Real-Time PCR Detection Systemno ROX
Opticon 2 – Continuous Fluorescence Detection Systemno ROX
Chromo4™ Four-Color Real-Time Detectorkein ROX

Eppendorf

qPCR CyclerROX necessary?
Mastercycler® ep realplex 4, Real-Time Thermal Cyclerno ROX
Mastercycler® ep realplex 4s, Real-Time Thermal Cyclerno ROX
Mastercycler® ep realplex, Real-Time Thermal Cyclerno ROX
Mastercycler® ep realplex s, Real-Time Thermal Cyclerno ROX
Mastercycler Nexusno ROX
Mastercycler Nexus gradientno ROX
Mastercycler Prono ROX
Mastercycler Pro Sno ROX
Mastercycler Pro 384no ROX

Roche Lightcycler

qPCR CyclerROX necessary?
LightCycler® 96 Systemno ROX
LightCycler® 480 Systemno ROX
LightCycler® 2.0 Instrumentno ROX
LightCycler® 1.5 Instrumentno ROX
LightCycler® 1536 Systemno ROX
LightCycler® Nano Systemno ROX

Analytik Jena

qPCR CyclerROX necessary?
qTowerlow ROX (optional)
qTower 2.0low ROX (optional)
qTower 2.2low ROX (optional)
qTower 3low ROX (optional)
qTower 3 84low ROX (optional)

Stratagene / Agilent

qPCR CyclerROX necessary?
Mx3000P® qPCR Systemlow ROX
Mx3005P® qPCR Systemlow ROX
Mx4000® qPCR Systemlow ROX

Qiagen

qPCR CyclerROX necessary?
Rotor-Gene™ Qlow ROX
Rotor-Gene™ 6000low ROX

Your Product Specialist

Jan Winter

Product Manager Genomics

PCR, qPCR, Magnetic Beads & CRISPR/Cas9

+49 (152) 3361 2537

jw@steinbrenner-laborsysteme.de