ROX compatibility of real-time qPCR machines

ROX is a non-intercalating reference dye, which can be added to the qPCR reaction when a compatible qPCR cycler is used. ROX can be used to normalize differences in the reaction volume, however, is not necessary in all cases.

Please check the table below if your qPCR cycler is ready to use ROX and at which concentration (low or high ROX).

Applied Biosystems

qPCR Cycler ROX necessary?
StepOne™ Real-Time PCR System high ROX
StepOnePlus™ Real-Time PCR System high ROX
7500 Real-Time PCR System low ROX
7500 Fast Real-Time PCR System low ROX
7500 Fast Dx Real-Time PCR Instrument low ROX
7500 Real-Time PCR System for Human Identification low ROX
7300 Real-Time PCR System High ROX
Viia™ 7 Real-Time PCR System low ROX
7900HT Fast Real-Time PCR System high ROX
OpenArray® Real-Time PCR Platform high ROX
QuantStudio™ 12K Flex system low ROX
PRISM® 7000 Sequencing Detection System high ROX
PRISM® 7700 Sequencing Detection System high ROX
PRISM® 7900 Sequencing Detection System high ROX
Gene Amp 5700 high ROX
Quantstudio™ 3 low Rox
Quantstudio™ 5 low Rox
Quantstudio™ 6 low Rox
Quantstudio™ 7 low Rox

Bio-Rad

qPCR Cycler ROX necessary?
CFX96 Touch™ Real-Time PCR Detection System no ROX
CFX384 Touch™ Real-Time PCR Detection System no ROX
Chromo4™ Four-Color Real-Time Detector no ROX
CFX Connect™ Real-Time PCR Detection System no ROX
iQ5 Real-Time PCR Detection System no ROX
MiniOpticon™ Real-Time PCR Detection System no ROX
Opticon 2 – Continuous Fluorescence Detection System no ROX
Chromo4™ Four-Color Real-Time Detector kein ROX

Eppendorf

qPCR Cycler ROX necessary?
Mastercycler® ep realplex 4, Real-Time Thermal Cycler no ROX
Mastercycler® ep realplex 4s, Real-Time Thermal Cycler no ROX
Mastercycler® ep realplex, Real-Time Thermal Cycler no ROX
Mastercycler® ep realplex s, Real-Time Thermal Cycler no ROX
Mastercycler Nexus no ROX
Mastercycler Nexus gradient no ROX
Mastercycler Pro no ROX
Mastercycler Pro S no ROX
Mastercycler Pro 384 no ROX

Roche Lightcycler

qPCR Cycler ROX necessary?
LightCycler® 96 System no ROX
LightCycler® 480 System no ROX
LightCycler® 2.0 Instrument no ROX
LightCycler® 1.5 Instrument no ROX
LightCycler® 1536 System no ROX
LightCycler® Nano System no ROX

Analytik Jena

qPCR Cycler ROX necessary?
qTower low ROX (optional)
qTower 2.0 low ROX (optional)
qTower 2.2 low ROX (optional)
qTower 3 low ROX (optional)
qTower 3 84 low ROX (optional)

Stratagene / Agilent

qPCR Cycler ROX necessary?
Mx3000P® qPCR System low ROX
Mx3005P® qPCR System low ROX
Mx4000® qPCR System low ROX

Qiagen

qPCR Cycler ROX necessary?
Rotor-Gene™ Q low ROX
Rotor-Gene™ 6000 low ROX

Your Product Specialist

Jan Winter

Product Manager Genomics

PCR, qPCR, Magnetic Beads & CRISPR/Cas9

+49 (152) 3361 2537

jw@steinbrenner-laborsysteme.de