Why this qPCR Seminar?
Quantitative PCR (qPCR) – or quantitative Real-time-PCR (qRT-PCR, RT-qPCR) – is used for applications in research and diagnostics. It is the method of choice for gene expression analysis, genotyping and NGS quantification. Although well established, there are quite a few myths about this method which indeed has its tricky parts. From our experience, most users are thus thankful for practical support and approved tips and information.
This is exactly what we are offering with our seminar “Best Practices for Quantitative Real-Time PCR“. It covers basic background as well as troubleshooting for typical applications and provides strategies on how to avoid common mistakes and how to validate and optimise your assay.
The seminar addresses lab workers and scientists, covering all knowledge levels. A certificate of attendance is available upon request.
The seminar will be held in English.
Upcoming Seminar
Currently, there is no public seminar
Previous Seminars
Tuesday, March 19, 2019
11:15 – 14:00
CMMC Building 66
(Center for Molecular Medicine Cologne)
“Großer Seminarraum EG”
Robert-Koch-Str. 21
50931 Köln
you will be familiar with the technical and scientific basics of qPCR
you will be able to better design an assay for your experiment
will know how to evaluate and validate the performance of a qPCR assay
you will be able to detect weaknesses and possible problems of your existing qPCR assay
will know how to optimise your assay
Technical and scientific basics
Typical use and applications of qPCR
Assay design
Primer design
Probe assay versus dye assay (e.g. SYBR Green)
Multiplexing: probes or SYBRGreen?
Assay validiation and performance check
Strategies for assay optimisation
Most common errors
Do’s and don’ts
Basic and advanced methods for data analysis
Slides
Lecturer
Jan Winter
Produktmanager Genomics
Application Specialist PCR, qPCR, NGS, Magnetic Beads und CRISPR/Cas9
+49 (152) 3361 2537
jw@steinbrenner-laborsysteme.de
Register now for the qPCR seminar
Fill in questionaire to attend the seminar