Why this qPCR Seminar?

Quantitative PCR (qPCR) – or quantitative Real-time-PCR (qRT-PCR, RT-qPCR) – is used for applications in research and diagnostics. It is the method of choice for gene expression analysis, genotyping and NGS quantification. Although well established, there are quite a few myths about this method which indeed has its tricky parts. From our experience, most users are thus thankful for practical support and approved tips and information.

This is exactly what we are offering with our seminar “Best Practices for Quantitative Real-Time PCR“. It covers basic background as well as troubleshooting for typical applications and provides strategies on how to avoid common mistakes and how to validate and optimise your assay.

The seminar addresses lab workers and scientists, covering all knowledge levels. A certificate of attendance is available upon request.

The seminar will be held in English.

Upcoming Seminar

Wednesday, February 19, 2020
13:15 – 16:00

free of charge

Biomedizinisches Centrum
(BMC)
Room N02.017

Großhaderner Str. 9,
82152 Planegg-Martinsried

Friday, April 3rd, 2020
10:00 – 13:00

Fee 329€ zzgl. MwSt

Analytika
Messe München, Halle 2, 1.OG

Additional Information and Registration:
Klinkner & Partner Analytica Specials

Currently, there are no further public seminars

Previous Seminars

Tuesday, March 19, 2019
11:15 – 14:00

CMMC Building 66
(Center for Molecular Medicine Cologne)
“Großer Seminarraum EG”

Robert-Koch-Str. 21
50931 Köln

After this seminar ...
  • you will be familiar with the technical and scientific basics of qPCR

  • you will be able to better design an assay for your experiment

  • will know how to evaluate and validate the performance of a qPCR assay

  • you will be able to detect weaknesses and possible problems of your existing qPCR assay

  • will know how to optimise your assay

Contents
  • Technical and scientific basics

  • Typical use and applications of qPCR

  • Assay design

  • Primer design

  • Probe assay versus dye assay (e.g. SYBR Green)

  • Multiplexing: probes or SYBRGreen?

  • Assay validiation and performance check

  • Strategies for assay optimisation

  • Most common errors

  • Do’s and don’ts

  • Basic and advanced methods for data analysis

Slides

qPCR Seminar Slides 2019 - Cologne
 15.53 MB
 16. April 2019
Version 1

Lecturer

Jan Winter
Produktmanager Genomics
Application Specialist PCR, qPCR, NGS, Magnetic Beads und CRISPR/Cas9

+49 (152) 3361 2537

jw@steinbrenner-laborsysteme.de

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